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COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal kub)

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Tus Kab Mob Khaus Viv-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) yog qhov ceev chromatographic immunoassay rau kev kuaj pom zoo ntawm cov tshuaj tiv thaiv kab mob rau COVID-19 hauv tib neeg cov ntshav, ntshav, lossis ntshav raws li kev pab cuam hauv kev kuaj pom muaj cov tshuaj tiv thaiv kab mob nruab nrab. rau COVID-19.

npe 1

Cov kab mob tshiab coronaviruses yog nyob rau β genus.COVID-19 yog tus kab mob ua pa hnyav heev.Tib neeg feem ntau raug mob.Tam sim no, cov neeg mob kis los ntawm tus kabmob tshiab no yog qhov tseem ceeb ntawm kev kis tus kabmob;Cov neeg mob asymptomatic kuj tuaj yeem yog qhov kis tau.Raws li kev tshawb nrhiav kev kis kabmob tam sim no, lub sijhawm incubation yog 1 txog 14 hnub, feem ntau yog 3 txog 7 hnub.Cov tsos mob tseem ceeb muaj xws li kub taub hau, qaug zog thiab hnoos qhuav.Qhov ntswg congestion, los ntswg qhov ntswg, mob caj pas, myalgia thiab raws plab yog pom nyob rau hauv ob peb kis.

Tus Kab Mob Khaus Viv-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) yog qhov kev sim sai uas siv kev sib xyaw ua ke ntawm S-RBD antigen coated coloured particles rau kev kuaj pom cov tshuaj tiv thaiv tsis zoo rau COVID-19 hauv tib neeg cov ntshav, ntshav, lossis ntshav.

npe 2

Tus Kab Mob Khaus Viv-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) yog ib daim nyias nyias raws li immunoassay rau kev kuaj pom cov tshuaj tiv thaiv tsis zoo rau COVID-19 hauv cov ntshav, ntshav lossis ntshav.Lub daim nyias nyias yog pre-coated nrog Angiotensin I Converting Enzyme 2 (ACE2) ntawm qhov chaw kuaj kab ntawm kab sawb.Thaum kuaj, tag nrho cov ntshav, ntshav los yog cov ntshav plasma reacts nrog S-RBD conjugated colloid kub.Qhov sib xyaw ua ke nce mus rau ntawm daim nyias nyias chromatographically los ntawm capillary ua kom hnov ​​​​mob nrog ACE2 ntawm daim nyias nyias thiab tsim cov kab xim.Kev tshwm sim ntawm cov kab xim no qhia txog qhov tshwm sim tsis zoo, thaum nws qhov tsis tuaj yeem qhia tau qhov txiaj ntsig zoo.Txhawm rau ua tus txheej txheem tswj hwm, cov kab xim yuav ib txwm hloov ntawm Xiav mus rau Liab hauv cheeb tsam tswj kab, qhia tias qhov ntim zoo ntawm cov qauv tau ntxiv thiab daim nyias nyias tau tshwm sim.

npe 3
Ib leeg packed test devices Txhua lub cuab yeej muaj ib txoj hlua nrog cov xim sib txuas thiab cov reactive reagents ua ntej kis ntawm cov cheeb tsam sib thooj
Pov tseg pipettes Rau kev ntxiv cov qauv siv
Tsis pub Phosphate buffered saline thiab preservative
Pob ntawv ntxig Rau kev qhia ua haujlwm
npe 4

Cov ntaub ntawv muab

●Test devices ●Droppers
● Tsis pub ●Package insert

Cov ntaub ntawv xav tau tab sis tsis muab

● Cov thawv ntim khoom ntim ● Timer
● Centrifuge  
npe 5

1. Rau kev kuaj mob hauv vitro tsuas yog siv xwb.
2. Tsis txhob siv tom qab hnub tas sij hawm teev nyob rau hauv pob.Tsis txhob siv qhov kev ntsuam xyuas yog tias lub hnab ntawv ci puas lawm.Tsis txhob rov siv cov kev ntsuam xyuas.
3. Cov tshuaj reagent extraction muaj cov tshuaj ntsev yog tias cov tshuaj tiv tauj rau ntawm daim tawv nqaij lossis qhov muag, yaug nrog dej ntau ntau.

4. Tsis txhob kis tus kab mob sib kis los ntawm kev siv lub thawv ntim khoom tshiab rau txhua tus qauv tau txais.
5. Nyeem tag nrho cov txheej txheem ua tib zoo ua ntej kuaj.
6. Tsis txhob noj, haus lossis haus luam yeeb nyob rau hauv qhov chaw uas cov qauv thiab cov khoom siv raug tuav.Kov tag nrho cov qauv zoo li lawv muaj cov kab mob sib kis.Ua raws li kev tiv thaiv kev tiv thaiv kab mob microbiological thoob plaws hauv cov txheej txheem thiab ua raws li cov txheej txheem rau kev pov tseg cov qauv.Hnav cov khaub ncaws tiv thaiv xws li cov tsho hauv chav kuaj, cov hnab looj tes pov tseg thiab tiv thaiv qhov muag thaum kuaj cov qauv.
7. Yog tias muaj tus kab mob tshiab coronaviruses raug xav tias yog raws li kev soj ntsuam kuaj mob tam sim no thiab cov kab mob sib kis tau pom zoo los ntawm tsoomfwv saib xyuas kev noj qab haus huv, cov qauv yuav tsum tau sau nrog cov kev tiv thaiv kab mob tsim nyog rau cov kab mob tshiab coronaviruses thiab xa mus rau lub xeev lossis lub chaw saib xyuas kev noj qab haus huv hauv nroog.Kab lis kev cai kab lis kev cai yuav tsum tsis txhob sim rau hauv cov xwm txheej no tshwj tsis yog muaj BSL 3+ kom tau txais thiab cov qauv kab lis kev cai.
8. Tsis txhob sib pauv lossis sib xyaw cov tshuaj reagents los ntawm ntau qhov sib txawv.
9. Cov av noo thiab kub tuaj yeem cuam tshuam qhov tshwm sim.
10. Cov khoom siv kuaj yuav tsum muab pov tseg raws li cov cai hauv zos.

npe 6

1. Cov khoom siv yuav tsum khaws cia ntawm 2-30 ° C kom txog rau thaum hnub tas sij hawm luam tawm ntawm lub hnab ntim khoom.
2. Kev sim yuav tsum nyob twj ywm hauv lub hnab ntim khoom kom txog thaum siv.
3. Tsis txhob khov.
4. Yuav tsum tau saib xyuas kom tiv thaiv cov khoom ntawm cov khoom siv los ntawm kev sib kis.Tsis txhob siv yog tias muaj pov thawj ntawm microbial paug los yog nag lossis daus.Kev kis kab mob lom ntawm cov khoom siv tshuaj, ntim lossis cov tshuaj reagents tuaj yeem ua rau cov txiaj ntsig tsis tseeb.

npe 7

Xav txog tej yam ntaub ntawv ntawm tib neeg keeb kwm li kis tau thiab tswj lawv siv cov txheej txheem biosafety.

Capillary Tag Nrho Cov Ntshav
Ntxuav tus neeg mob txhais tes ces cia kom qhuav.Massage tes yam tsis tau kov qhov puncture.Puncture daim tawv nqaij nrog ib tug sterile lancet.So thawj lub cim ntawm ntshav.Maj mam rub tes ntawm dab teg mus rau xib teg mus rau ntiv tes los ua ib qho kev sib npaug ntawm cov ntshav hla ntawm qhov chaw.Ntxiv cov Fingerstick Whole Blood specimen rau lub cuab yeej kuaj los ntawm kev siv lub raj capillary lossis dai tee.

venous tag nrho cov ntshav:
Sau cov ntshav coj mus rau hauv lub paj noob hlis, xiav lossis ntsuab sau cov raj (muaj EDTA, citrate lossis heparin, raws li hauv Vacutainer®) los ntawm veinpuncture.

Plasma
Sau cov ntshav coj mus rau hauv lub paj noob hlis, xiav lossis ntsuab sau cov raj (muaj EDTA, citrate lossis heparin, raws li hauv Vacutainer®) los ntawm veinpuncture.Cais cov ntshav los ntawm centrifugation.Ua tib zoo tshem cov plasma mus rau hauv lub raj tshiab pre-labeled.

Serum
Sau cov ntshav mus rau hauv cov hlab ntshav liab (tsis muaj cov tshuaj tiv thaiv coagulants hauv Vacutainer®) los ntawm veinpuncture.Cia cov ntshav txhaws.Cais cov ntshav los ntawm centrifugation.Ua tib zoo tshem cov ntshav mus rau hauv lub raj tshiab pre-labeled.
Ntsuas cov qauv kom sai li sai tau tom qab sau.Khaws cov qauv ntawm 2 ° C-8 ° C yog tias tsis kuaj tam sim.
Khaws cov qauv ntawm 2 ° C-8 ° C mus txog 5 hnub.Cov qauv yuav tsum tau khov ntawm -20 ° C kom khaws cia ntev dua.
Zam ntau lub voj voog khov-thaw.Ua ntej kev sim, nqa cov qauv khov rau hauv chav sov maj mam thiab sib tov maj mam.Cov qauv uas muaj qhov pom ntawm qhov teeb meem yuav tsum tau qhia meej los ntawm centrifugation ua ntej kuaj.Tsis txhob siv cov qauv uas qhia txog tag nrho cov lipemia, tag nrho hemolysis lossis turbidity txhawm rau kom tsis txhob cuam tshuam rau kev txhais lus.

npe 8

Nqa cov qauv thiab cov khoom kuaj mus rau hauv chav tsev kub Sib tov cov qauv zoo ua ntej kev soj ntsuam ib zaug thawed.Muab cov cuab yeej kuaj rau ntawm qhov chaw huv, tiaj tus.

Rau capillary tag nrho cov ntshav kuaj:
Siv lub raj capillary: Sau lub raj capillary thiabHloov li 50µL (los yog 2 tee) ntawm cov ntiv tes tag nrho cov ntshavcov qauv mus rau qhov hnoos qeev (S) ntawm cov khoom kuaj, tom qab ntawd ntxiv1 poob (li 30 µL)ntawmQauv Diluenttam sim ntawd mus rau hauv tus qauv zoo.

Rau tag nrho cov ntshav kuaj:
Sau lub dropper nrog cov qauv ceshloov 2 tee (li 50 µL)ntawm cov qauv rau hauv qhov qauv zoo.Ua kom paub tseeb tias tsis muaj cua npuas.Ceshloov 1 poob (txog 30 µL)ntawm Sample Diluent tam sim ntawd mus rau hauv cov qauv zoo.

Rau Plasma / Serum qauv:
Sau lub dropper nrog cov qauv ceshloov 1 poob (li 25 µL)ntawm cov qauv rau hauv qhov qauv zoo.Ua kom paub tseeb tias tsis muaj cua npuas.Ceshloov 1 poob (txog 30 µL) ntawm Sample Diluent tam sim ntawd mus rau hauv cov qauv zoo.
Teem lub timer.Nyeem qhov tshwm sim ntawm 15 feeb.Tsis txhob nyeem cov txiaj ntsig tom qab20 feeb.Txhawm rau kom tsis txhob muaj kev ntxhov siab, muab pov tseg lub cuab yeej kuaj tom qab txhais cov txiaj ntsig

npe 9

POSITIVE RESULT:
img ua

 

Tsuas yog ib qho xim dawb tshwm nyob rau hauv cheeb tsam tswj (C).Tsis muaj cov xim dawb tshwm nyob rau hauv cheeb tsam xeem (T).

NEGATIVE RESULT:
ib img1

 

Ob lub xim dawb tshwm rau ntawm daim nyias nyias.Ib pawg tshwm nyob rau hauv cheeb tsam tswj (C) thiab lwm pab pawg tshwm nyob rau hauv cheeb tsam xeem (T).
* CEEB TOOM: Qhov kev siv ntawm cov xim nyob rau hauv cheeb tsam kab xeem yuav txawv nyob ntawm seb qhov concentration ntawm neutralizing antibodies rau COVID-19 nyob rau hauv cov qauv.Yog li ntawd, txhua qhov ntxoov ntxoo ntawm cov xim hauv cheeb tsam kab xeem yuav tsum raug xam tias tsis zoo.

 

INVALID RESULT:
img2 ua

 

 

 

Tswj band tsis tshwm sim.Cov txiaj ntsig los ntawm ib qho kev xeem uas tsis tau tsim ib pawg tswj hwm ntawm lub sijhawm nyeem ntawv yuav tsum raug muab pov tseg.Thov tshuaj xyuas cov txheej txheem thiab rov ua dua nrog kev sim tshiab.Yog tias muaj teeb meem tshwm sim, txiav tsis siv cov khoom siv tam sim ntawd thiab tiv tauj koj tus neeg muag khoom hauv zos.
npe 10

1. Internal Control:Qhov kev ntsuam xyuas no muaj cov yam ntxwv ntawm kev tswj hwm, C band.Cov kab C tsim tom qab ntxiv cov qauv thiab cov qauv diluent.Txwv tsis pub, tshuaj xyuas tag nrho cov txheej txheem thiab rov sim dua nrog lub cuab yeej tshiab.
2. Sab nraud tswj:Kev Xyaum Ua Haujlwm Zoo pom zoo kom siv cov kev tswj xyuas sab nraud, qhov zoo thiab qhov tsis zoo (muab raws li kev thov), kom paub tseeb tias qhov ua tau zoo ntawm qhov kev xeem.


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