Npaj siv

Dengue NS1 Ag-IgG/IgM Combo Test yog ib qho tshuaj tiv thaiv kab mob sai sai rau kev kuaj pom zoo ntawm cov tshuaj tiv thaiv kab mob (IgG thiab IgM) thiab tus kab mob dengue NS1 antigen rau tus kab mob dengue hauv Serum lossis Plasma los pab kuaj mob tus kab mob Dengue.

[SUMMARY]

Dengue fever yog ib yam kab mob uas kis tau los ntawm tus kab mob dengue los ntawm yoov tshaj cum.Kev kis tus kab mob dengue tuaj yeem ua rau tus kab mob kis tau zoo, mob npaws dengue, dengue hemorrhagic fever, dengue hemorrhagic fever.Cov tsos mob tshwm sim ntawm tus kab mob dengue muaj xws li tshwm sim tam sim ntawd, kub taub hau, mob taub hau, mob hnyav, pob txha thiab mob pob qij txha, daim tawv nqaij ua pob khaus, los ntshav nyiam, lymph node o, txo cov ntshav dawb suav, thrombocytopenia thiab lwm yam hauv qee cov neeg mob.Tus kab mob no yeej yog nyob rau hauv cheeb tsam kub thiab subtropical qhov chaw, vim hais tias tus kab mob no kis los ntawm Aides yoov tshaj cum, yog vim li cas muaj koob meej muaj raws caij nyoog, nyob rau hauv txhua txhua xyoo feem ntau nyob rau hauv lub Tsib Hlis ~ Kaum ib hlis, ncov yog nyob rau hauv Lub Xya hli ntuj ~ Cuaj hlis.Hauv thaj chaw tshiab muaj kabmob kis, cov pejxeem feem ntau raug cuam tshuam, tab sis qhov tshwm sim feem ntau yog cov neeg laus, nyob rau hauv thaj chaw endemic, qhov tshwm sim feem ntau yog menyuam yaus.

Txoj Cai

Lub Dengue NS1 Ag-IgG / IgM Combo Test yog ib qho zoo ntawm daim nyias nyias strip raws li immunoassay rau kev kuaj pom tus kab mob dengue antibodies (IgG thiab IgM) thiab tus kab mob dengue NS1 antigen hauv Serum lossis Plasma.

Rau IgG / IgM Test: Cov cuab yeej ntsuas muaj xws li: 1) burgundy coloured conjugate ncoo uas muaj dengue recombinant hnab ntawv antigens conjugated nrog Colloid kub (dengue conjugates), 2) nitrocellulose membrane sawb uas muaj ob kab xeem (T1 thiab T2 kab) thiab kab tswj (C kab).T1 kab yog pre-coated nrog cov tshuaj tiv thaiv rau kev tshawb pom ntawm IgM los tiv thaiv tus mob khaub thuas, T2 kab yog coated nrog cov tshuaj tiv thaiv rau kev tshawb pom ntawm IgG anti-dengue.Thaum lub ntim ntim txaus ntawm cov qauv kuaj tau muab xa mus rau hauv cov qauv zoo ntawm cov ntawv xeem, cov qauv txav los ntawm kev ua haujlwm capillary thoob plaws lub cassette.IgG anti-dengue, yog muaj nyob rau hauv cov qauv, yuav khi rau cov dengue conjugates.Lub immunocomplex yog ces ntes tau los ntawm cov reagent pre-coated ntawm T2 band, tsim ib tug burgundy xim T2 kab, qhia tau hais tias ib tug dengue IgG zoo kuaj tau thiab qhia ib tug tsis ntev los no los yog rov kis kab mob.IgM anti-dengue yog muaj nyob rau hauv cov qauv yuav khi rau cov dengue conjugates.Lub immunocomplex yog tom qab ntawd ntes tau los ntawm cov reagent coated ntawm T1 kab, tsim ib tug burgundy xim T1 kab, qhia tau hais tias ib tug dengue IgM zoo kuaj tau thiab qhia ib tug tshiab kab mob.Tsis muaj ib kab T (T1 thiab T2) qhia tias qhov tshwm sim tsis zoo.

Rau NS1 Test: Nyob rau hauv cov txheej txheem kev sim no, anti-Dengue NS1 antibody yog immobilized nyob rau hauv lub xeem kab cheeb tsam ntawm lub cassette.Tom qab tag nrho Cov Ntshav / Ntshav / Plasma cov qauv tau muab tso rau hauv cov qauv zoo, nws hnov ​​​​mob nrog anti-Dengue NS1 cov tshuaj tiv thaiv kab mob coated uas tau siv rau hauv cov ntaub qhwv.Qhov sib tov no migrate chromatographically raws li qhov ntev ntawm lub xeem strip thiab cuam tshuam nrog immobilized anti-Dengue NS1 antibody.Yog tias cov qauv muaj tus kab mob dengue NS1 antigen, ib kab xim xim yuav tshwm sim hauv cheeb tsam kab kuaj pom tias muaj txiaj ntsig zoo.Yog tias cov qauv no tsis muaj tus kab mob dengue NS1 antigen, ib kab xim yuav tsis tshwm sim hauv cheeb tsam no qhia tias qhov tshwm sim tsis zoo.

Txhawm rau ua raws li kev tswj hwm txheej txheem, cov kab xim xim yuav ib txwm tshwm sim ntawm thaj chaw tswj kab uas qhia tias qhov ntim ntawm cov qauv raug muab ntxiv thiab daim nyias nyias tau tshwm sim.

Cia thiab ruaj khov

Khaws cia li ntim rau hauv lub hnab ntim khoom ntawm chav tsev kub lossis lub tub yees (4-30 ℃ lossis 40-86 ℉).Cov cuab yeej kuaj no ruaj khov los ntawm hnub tas sij hawm luam tawm ntawm lub hnab ntim khoom.

Qhov kev sim yuav tsum nyob twj ywm hauv lub hnab kaw kom txog thaum siv.

Kev Sau thiab Kev Npaj

1. Qhov Kev Ntsuas Dengue NS1 Ag-IgG/IgM Combo Test tuaj yeem ua tau siv rau hauv ntshav lossis ntshav ntshav.

2. Txhawm rau sau tag nrho cov ntshav, ntshav ntshav lossis ntshav plasma raws li cov txheej txheem kuaj mob tsis tu ncua.

3. Kev ntsuam xyuas yuav tsum tau ua tam sim tom qab sau cov qauv.Tsis txhob tso cov qauv ntawm chav tsev kub rau lub sijhawm ntev.Rau kev khaws cia ntev, cov qauv yuav tsum khaws cia hauv qab-20 ℃.

4. Nqa cov qauv mus rau chav tsev kub ua ntej kev sim.Cov qauv khov yuav tsum tau thawed tag nrho thiab sib tov zoo ua ntej kuaj.Cov khoom yuav tsum tsis txhob khov thiab thawed ntau zaus.

Cov txheej txheem xeem

Tso cai rau kev sim, piv txwv, tsis thiab / lossis kev tswj kom ncav cuag chav sov 15-30 ℃ (59-86 ℉) ua ntej kuaj.

1. Nqa lub hnab rau hauv chav sov ua ntej qhib nws.Tshem tawm cov cuab yeej kuaj ntawm lub hnab ntim thiab siv nws sai li sai tau.Muab cov cuab yeej kuaj rau ntawm qhov chaw huv thiab qib.

2. Rau IgG / IgM Test : Tuav lub dropper vertically thiab hloov 1 poob ntawm cov qauv (kwv yees li 10μl) mus rau cov qauv zoo (S) ntawm cov cuab yeej kuaj, tom qab ntawd ntxiv 2 tee ntawm qhov tsis sib xws (kwv yees li 70μl) thiab pib lub sijhawm.Saib daim duab hauv qab no.

3. Rau NS1 Test: Tuav lub dropper vertically thiab hloov 8 ~ 10 tee ntawm cov ntshav los yog ntshav (kwv yees li 100μl) rau cov qauv zoo (S) ntawm cov cuab yeej kuaj, ces pib lub sijhawm.Saib daim duab hauv qab no.

4. Tos kom pom cov kab uas muaj xim.Nyeem cov txiaj ntsig ntawm 15 feeb.Tsis txhob txhais cov txiaj ntsig tom qab 20 feeb.

Kev txhais cov ntsiab lus

Qhov zoo:

Rau IgG / IgM Test: Cov kab tswj thiab tsawg kawg ib kab kev sim tshwm sim ntawm daim nyias nyias.Cov tsos mob ntawm T2 kuaj kab qhia tias muaj cov kab mob dengue tshwj xeeb IgG cov tshuaj tiv thaiv.Cov tsos mob ntawm T1 kuaj kab qhia tias muaj cov kab mob dengue tshwj xeeb IgM cov tshuaj tiv thaiv.Thiab yog tias ob qho tib si T1 thiab T2 kab tshwm sim, nws qhia tau tias muaj ob qho tib si dengue tshwj xeeb IgG thiab IgM cov tshuaj tiv thaiv.Qhov qis dua cov tshuaj tiv thaiv kab mob yog, qhov tsis muaj zog ntawm cov kab mob tshwm sim.

Rau NS1 Test: Ob kab tshwm.Ib kab yuav tsum ib txwm tshwm sim hauv cheeb tsam kab tswj (C), thiab lwm qhov pom tseeb xim kab yuav tsum tshwm sim hauv cheeb tsam kab xeem.

Tsis zoo:

Ib kab xim muaj xim tshwm nyob rau hauv cheeb tsam tswj (C).Tsis muaj cov kab xim pom tseeb tshwm hauv cheeb tsam kab xeem.

Invalid: Tswj kab tsis tshwm sim.Cov khoom ntim tsis txaus lossis cov txheej txheem tsis raug yog cov laj thawj feem ntau rau kev tswj kab tsis ua haujlwm.Tshawb xyuas cov txheej txheem thiab rov ua qhov kev sim nrog ib qho kev sim tshiab.Yog tias muaj teeb meem tshwm sim, txiav tawm siv cov khoom kuaj tam sim ntawd thiab tiv tauj koj tus neeg muag khoom hauv zos.