Cov kws tshawb fawb Dutch muab CRISPR thiab bioluminescence hauv kev sim rau kev simkis kab mob

Ib qho kev tsim tshiab nocturnal protein tuaj yeem ua kom sai thiab yooj yim rau kev kuaj mob ntawm cov kab mob, raws li cov kws tshawb fawb hauv Netherlands.
Lawv txoj kev tshawb fawb, luam tawm hnub Wednesday hauv ACS Cov Ntawv Tshaj Tawm, piav qhia txog kev nkag siab, ib kauj ruam rau kev soj ntsuam cov kab mob nucleic acids sai thiab lawv cov tsos siv ci ntsa iab xiav lossis ntsuab proteins.
Kev txheeb xyuas cov kab mob los ntawm kev kuaj xyuas lawv cov ntiv tes nucleic acid yog lub tswv yim tseem ceeb hauv kev kuaj mob, kev tshawb fawb biomedical, thiab kev saib xyuas zaub mov thiab ib puag ncig kev nyab xeeb.Kev siv ntau yam polymerase saw cov tshuaj tiv thaiv (PCR) cov kev ntsuam xyuas yog cov rhiab heev, tab sis xav tau cov qauv kev npaj zoo lossis kev txhais cov txiaj ntsig, ua rau lawv ua tsis tau zoo rau qee qhov chaw kho mob lossis chaw muaj peev txheej txwv.
Cov pab pawg no los ntawm Netherlands yog qhov tshwm sim ntawm kev sib koom tes ntawm cov kws tshawb fawb los ntawm cov tsev kawm qib siab thiab tsev kho mob txhawm rau txhim kho txoj kev kuaj mob sai, nqa tau yooj yim thiab yooj yim siv nucleic acid uas tuaj yeem siv rau ntau qhov chaw.
Lawv tau txais kev tshoov siab los ntawm cov hluav taws kub hnyiab, hluav taws kub hnyiab, thiab cov hnub qub me me ntawm cov dej phytoplankton, txhua tus siv los ntawm qhov tshwm sim hu ua bioluminescence.Cov nyhuv ci ntsa iab hauv qhov tsaus ntuj no yog tshwm sim los ntawm cov tshuaj lom neeg cov tshuaj tiv thaiv nrog cov protein luciferase.Cov kws tshawb fawb tau koom nrog cov proteins luciferase rau hauv cov sensors uas tawm lub teeb los pab kev soj ntsuam thaum lawv pom lub hom phiaj.Thaum qhov no ua rau cov sensors zoo tagnrho rau kev kuaj xyuas qhov chaw saib xyuas, lawv tam sim no tsis muaj qhov siab xav tau rau kev kuaj mob kuaj mob.Thaum CRISPR gene kho txoj kev tuaj yeem muab lub peev xwm no, nws yuav tsum tau muaj ntau cov kauj ruam thiab cov cuab yeej tshwj xeeb ntxiv txhawm rau txheeb xyuas cov teeb liab tsis muaj zog uas tuaj yeem muaj nyob hauv cov qauv nyuaj, nrov nrov.
Cov kws tshawb fawb tau pom ib txoj hauv kev los ua ke CRISPR cov protein muaj feem xyuam nrog lub teeb liab bioluminescent uas tuaj yeem kuaj pom nrog lub koob yees duab digital yooj yim.Txhawm rau kom paub tseeb tias muaj RNA lossis DNA qauv txaus rau kev tshuaj xyuas, cov kws tshawb fawb tau ua cov tshuaj recombinase polymerase amplification (RPA), cov txheej txheem yooj yim uas ua haujlwm ntawm qhov kub thiab txias ntawm ib ncig ntawm 100 ° F.Lawv tau tsim lub platform tshiab hu ua Luminescent Nucleic Acid Sensor (LUNAS), uas ob lub CRISPR / Cas9 cov proteins tshwj xeeb rau qhov sib txawv ntawm cov kab mob genome, txhua tus muaj ib qho tshwj xeeb luciferase fragment txuas rau lawv saum toj no.
Thaum cov kab mob kis tshwj xeeb uas cov neeg tshawb xyuas tau tshuaj xyuas yog tam sim no, ob CRISPR / Cas9 proteins khi rau lub hom phiaj nucleic acid ib ntus;lawv ua nyob ze, tso cai rau cov protein luciferase tsis zoo los tsim thiab tawm lub teeb xiav nyob rau hauv muaj cov tshuaj substrate..Txhawm rau suav rau cov substrate noj hauv cov txheej txheem no, cov kws tshawb fawb tau siv cov tshuaj tiv thaiv uas tawm teeb ntsuab.Ib lub raj uas hloov xim ntawm ntsuab mus rau xiav qhia tau tias muaj txiaj ntsig zoo.
Cov kws tshawb fawb tau sim lawv lub platform los ntawm kev tsim RPA-LUNAS kev soj ntsuam, uas kuaj pomSARS-CoV-2 RNAtsis muaj kev sib cais RNA txaus ntshai, thiab ua kom pom nws qhov kev kuaj mob ntawm nasopharyngeal swab kuaj los ntawmCOVID 19cov neeg mob.RPA-LUNAS ua tiav kuaj pom SARS-CoV-2 nyob rau hauv 20 feeb hauv cov qauv nrog RNA viral load tsawg li 200 daim ntawv / μL.
Cov kws tshawb fawb ntseeg tias lawv qhov kev ntsuam xyuas tuaj yeem pom tau yooj yim thiab pom tau ntau yam kab mob.Lawv tau sau tias "RPA-LUNAS yog qhov txaus nyiam rau qhov chaw saib xyuas kab mob sib kis," lawv sau.